5O3R
Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 64.425, 64.425, 80.737 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.700 - 1.900 |
| R-factor | 0.17809 |
| Rwork | 0.177 |
| R-free | 0.20686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o3p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.741 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.700 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.851 |
| Number of reflections | 29560 | 4763 |
| <I/σ(I)> | 20.3 | 2.11 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8.22 | 7.22 |
| CC(1/2) | 0.999 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | Protein solution containing 19 mg/ml SbtB in 50 mM Tris.HCl (pH 8.0), 250 mM NaCl, 3mM AMP mixed 1:1 with reservoir solution of Molecular Dimensions Morpheus screen condition A2 |
