5O3Q
Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with cyclic AMP (cAMP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 64.386, 64.386, 81.459 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.890 - 1.750 |
| R-factor | 0.17094 |
| Rwork | 0.170 |
| R-free | 0.19296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o3p |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.130 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.890 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.045 | 0.961 |
| Number of reflections | 38069 | 6125 |
| <I/σ(I)> | 22.14 | 2.13 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.16 | 7.04 |
| CC(1/2) | 0.999 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293.15 | Protein solution containing 19 mg/ml SbtB in 50 mM Tris.HCl (pH 8.0), 250 mM NaCl, 2mM cAMP mixed 1:1 with reservoir solution of Molecular Dimensions Morpheus screen condition G2 |
