5O3Q

Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with cyclic AMP (cAMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	64.386, 64.386, 81.459
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.890 - 1.750
R-factor	0.17094
Rwork	0.170
R-free	0.19296
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o3p
RMSD bond length	0.019
RMSD bond angle	2.130
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.890	1.860
High resolution limit [Å]	1.750	1.750
Rmerge	0.045	0.961
Number of reflections	38069	6125
<I/σ(I)>	22.14	2.13
Completeness [%]	99.9	99.7
Redundancy	7.16	7.04
CC(1/2)	0.999	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293.15	Protein solution containing 19 mg/ml SbtB in 50 mM Tris.HCl (pH 8.0), 250 mM NaCl, 2mM cAMP mixed 1:1 with reservoir solution of Molecular Dimensions Morpheus screen condition G2