5O3P

Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in Apo state, trigonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	64.351, 64.351, 82.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.160 - 1.750
R-factor	0.16525
Rwork	0.164
R-free	0.19377
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dfe
RMSD bond length	0.020
RMSD bond angle	1.980
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.160	1.850
High resolution limit [Å]	1.750	1.750
Rmerge	0.051	0.796
Number of reflections	38551	6130
<I/σ(I)>	18.62	2.07
Completeness [%]	99.7	97.9
Redundancy	7.2	7.14
CC(1/2)	0.999	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.2	293.15	Protein solution containing 19 mg/ml SbtB in 50 mM Tris.HCl (pH 8.0), 250 mM NaCl mixed 1:1 with reservoir solution composed of 0.1 M phosphate-citrate pH 4.2, and 40 % (w/v) PEG300.