5O3K
Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus with 1 mM copper sulfate on growth medium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.968630 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.027, 94.957, 116.456 |
| Unit cell angles | 89.88, 90.07, 91.70 |
Refinement procedure
| Resolution | 49.585 - 2.100 |
| R-factor | 0.2046 |
| Rwork | 0.196 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lhl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.054 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 176795 |
| <I/σ(I)> | 4.57 |
| Completeness [%] | 91.8 |
| Redundancy | 2.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 23% PEG 4000, 0,05M TRIS-HCL |
