5O3J

Crystal structure of TIA-1 RRM2 in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99
Spacegroup name	P 65
Unit cell lengths	44.310, 44.310, 85.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.020 - 2.970
R-factor	0.25387
Rwork	0.251
R-free	0.31160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bs9
RMSD bond length	0.006
RMSD bond angle	0.906
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.020
High resolution limit [Å]	2.970
Rmerge	0.087
Number of reflections	1980
<I/σ(I)>	15.34
Completeness [%]	98.0
Redundancy	7.8
CC(1/2)	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	50mM MES pH6, 5mM MgSO4, 5% PEG4000