5O36

Japanese encephalitis virus non-structural protein 1' C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	I 21 21 21
Unit cell lengths	50.320, 77.940, 163.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.750 - 2.600
R-factor	0.16994
Rwork	0.167
R-free	0.22431
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oie
RMSD bond length	0.016
RMSD bond angle	1.741
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.750	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.200	1.413
Rpim	0.141	1.030
Number of reflections	10247	1203
<I/σ(I)>	7.1	2.1
Completeness [%]	99.9	99.9
Redundancy	5.3	5.2
CC(1/2)	0.983	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.0 M Ammonium sulfate, 5% (v/v) propanol