5O21
Crystal structure of WNK3 kinase domain in a monophosphorylated state with chloride bound in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.77 |
| Spacegroup name | P 32 |
| Unit cell lengths | 117.410, 117.410, 46.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.705 - 2.060 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.514 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.710 |
| High resolution limit [Å] | 2.060 |
| Number of reflections | 43853 |
| <I/σ(I)> | 14.5 |
| Completeness [%] | 99.9 |
| Redundancy | 32.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 22.5% PEG Smear Broad, 0.1 M sodium/potassium tartrate, 10% ethylene glycol, 0.1 M cacodylate pH 5.5 |
