5O1W
Structure of Nrd1 RNA binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-14 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.991, 60.991, 157.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.900 - 2.300 |
| R-factor | 0.20796 |
| Rwork | 0.207 |
| R-free | 0.23297 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.306 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.140 | |
| High resolution limit [Å] | 2.300 | |
| Number of reflections | 383024 | |
| <I/σ(I)> | 32.1 | |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 24.5 | 26.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 12% PEG 8000, 0.1 M Bicine pH 9 |
