5O1P
Crystal structure of human aminoadipate semialdehyde synthase, saccharopine dehydrogenase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.928190 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 91.462, 91.462, 150.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.030 - 1.900 |
| R-factor | 0.31128 |
| Rwork | 0.311 |
| R-free | 0.32284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l76 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.008 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.112 | |
| Number of reflections | 51064 | 3234 |
| <I/σ(I)> | 12.2 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.2 | 12.6 |
| CC(1/2) | 0.973 | 0.204 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 286 | 0.1M tris pH 7.5 -- 0.33M sodium malonate -- 19.5% PEG3350 |
