5O1M
Structure of Latex Clearing Protein LCP in the closed state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.73672 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.863, 56.544, 63.861 |
| Unit cell angles | 74.25, 86.07, 71.04 |
Refinement procedure
| Resolution | 61.450 - 2.200 |
| R-factor | 0.1769 |
| Rwork | 0.174 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.007 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.450 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.065 | |
| Rpim | 0.039 | 0.180 |
| Number of reflections | 30914 | |
| <I/σ(I)> | 12.3 | 3.8 |
| Completeness [%] | 87.2 | 83.7 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.997 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298.15 | 16 % (w/v) PEG 3350, 0.2 M L-proline, 0.1 M HEPES/NaOH |
