5O1L
Structure of Latex Clearing Protein LCP in the open state with bound imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.758, 62.796, 64.416 |
| Unit cell angles | 85.43, 66.06, 74.16 |
Refinement procedure
| Resolution | 60.370 - 1.480 |
| R-factor | 0.16698 |
| Rwork | 0.166 |
| R-free | 0.18857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.370 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.086 | |
| Rpim | 0.053 | 0.358 |
| Number of reflections | 117382 | |
| <I/σ(I)> | 8.1 | 2.1 |
| Completeness [%] | 90.0 | 89.2 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.994 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 4 % (w/v) PEG 4000, 0.2 M Malate/Imidazole |
