5O19

Japanese encephalitis virus non-structural protein 1 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	I 21 21 21
Unit cell lengths	49.418, 78.244, 163.176
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.300 - 2.100
R-factor	0.19074
Rwork	0.189
R-free	0.22772
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oie
RMSD bond length	0.016
RMSD bond angle	1.785
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.103	0.907
Rpim	0.045	0.383
Number of reflections	18896	2701
<I/σ(I)>	11.5	2.3
Completeness [%]	99.8	99.6
Redundancy	6.3	6.5
CC(1/2)	0.998	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1 M Ammonium sulphate and 0.1 M MES pH 5.5