5O0V

crystal structure of E. coli GAP-DH by fortuitous crystallization as an impurity from a solution of human liver FBPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97940
Spacegroup name	I 41 2 2
Unit cell lengths	121.384, 121.384, 157.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.916 - 2.400
R-factor	0.1557
Rwork	0.154
R-free	0.18890
RMSD bond length	0.007
RMSD bond angle	0.853
Data reduction software	DENZO
Data scaling software	SADABS
Phasing software	COMO
Refinement software	PHENIX ((1.11_2558: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.920	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	23078	2213
<I/σ(I)>	12.13	1.33
Completeness [%]	99.0	92
Redundancy	11.62	3.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	unknown