5O04

GII.10 Vietnam 026 norovirus protruding domain in complex with Nanobody Nano-26 and Nano-85

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	C 1 2 1
Unit cell lengths	167.250, 91.480, 118.120
Unit cell angles	90.00, 127.12, 90.00

Refinement procedure

Resolution	47.978 - 2.300
R-factor	0.2131
Rwork	0.212
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.674
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.978	47.978	2.440
High resolution limit [Å]	2.300	6.840	2.300
Rmerge	0.079	0.031	0.592
Rmeas	0.097	0.039	0.727
Number of reflections	58206	2177	9174
<I/σ(I)>	10.74	29.57	2.29
Completeness [%]	91.7	86.1	90.1
Redundancy	2.823	2.765	2.733
CC(1/2)	0.996	0.997	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M sodium citrate, 40% PEG600