5O01
Crystal structure of BtubC (BKLC) from P. vanneervenii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.006 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 119.734, 119.734, 239.366 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.18863 |
| Rwork | 0.186 |
| R-free | 0.23665 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.716 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.108 | 0.674 |
| Rpim | 0.054 | 0.330 |
| Number of reflections | 21275 | |
| <I/σ(I)> | 10.8 | 2.7 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 4.9 | 5.2 |
| CC(1/2) | 0.996 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 50 mM CHES/NaOH, 500 mM NaCl, 1 mM TCEP, 5% glycerol, pH 9.0 |
