5NYW
Anbu (ancestral beta-subunit) from Yersinia bercovieri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.535, 285.371, 179.213 |
| Unit cell angles | 90.00, 91.78, 90.00 |
Refinement procedure
| Resolution | 49.185 - 2.501 |
| R-factor | 0.1831 |
| Rwork | 0.183 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nyg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.253 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 1.078 |
| Number of reflections | 325849 | |
| <I/σ(I)> | 14.63 | 2.06 |
| Completeness [%] | 98.9 | 95.1 |
| Redundancy | 5.78 | 5.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 291 | 0.1M BIS-TRIS PROPANE/HCL (pH 6.9); 18% w/v PEG 3350; 7.5% v/v ETHYLENE GLYCOL; 0.2M NA2SO4 |
