5NYR
Anbu from Hyphomicrobium sp. strain MC1 -SG: R3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 174.490, 174.490, 39.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.1836 |
| Rwork | 0.182 |
| R-free | 0.21367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nyf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.995 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.532 |
| Number of reflections | 29744 | |
| <I/σ(I)> | 9.8 | 2.5 |
| Completeness [%] | 98.5 | 99.7 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298.15 | 0.05 M ammonium sulfate, 0.05 M Bis-Tris, 30 % pentaerythritol propoxylate |
