5NYO
Crystal structure of an atypical poplar thioredoxin-like2.1 variant in dimeric form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.547, 62.547, 138.415 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.400 - 2.250 |
| R-factor | 0.2098 |
| Rwork | 0.207 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | wild-type Thioredoxin-like protein 2.1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.068 | 1.078 |
| Rpim | 0.035 | 0.552 |
| Number of reflections | 13773 | 1242 |
| <I/σ(I)> | 14.9 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 8.8 |
| CC(1/2) | 0.998 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M sodium formate, 20% (w/v) PEG3350 |
