5NYM
Crystal structure of the atypical poplar thioredoxin-like2.1 in reduced state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2012-05-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.546, 52.946, 59.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.450 - 1.400 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | thioredoxin-like2.1 oxidized form |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.450 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.021 | 0.555 |
| Rpim | 0.020 | 0.512 |
| Number of reflections | 28141 | 1268 |
| <I/σ(I)> | 22.1 | 2 |
| Completeness [%] | 95.4 | 87.9 |
| Redundancy | 3.6 | 3.4 |
| CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Na acetate, 0.2M lithium sulfate, 0.1M Hepes 7.5, 25% (w/w) PEG 4K |
