5NWS
Crystal structure of saAcmM involved in actinomycin biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.500 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 79.254, 129.539, 111.611 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.037 - 2.227 |
| R-factor | 0.1799 |
| Rwork | 0.178 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a4h |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.118 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.040 |
| High resolution limit [Å] | 2.227 |
| Number of reflections | 54229 |
| <I/σ(I)> | 11.8 |
| Completeness [%] | 99.7 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 17.5% (w/v) polyethylene glycol 6000 100 mM Tris/HCl pH 8.5 |
