5NWI

14-3-3c in complex with CPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-02
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 65 2 2
Unit cell lengths	110.177, 110.177, 136.691
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.043 - 2.350
R-factor	0.1983
Rwork	0.196
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o9d
RMSD bond length	0.013
RMSD bond angle	1.151
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.710	2.430
High resolution limit [Å]	2.350	2.350
Rpim	0.045	0.365
Number of reflections	21014	2044
<I/σ(I)>	15.2	5.1
Completeness [%]	100.0	100
Redundancy	39	27.7
CC(1/2)	0.971	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	30% PEG 400, 0.2 M ammonium acetate pH 7.0, 0.1 M sodium citrate pH 4.4, 10mM DTT and 5% glycerol