5NWA

Crystal structure of the complex of Tdp1 with duplex DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.92819
Spacegroup name	P 21 21 2
Unit cell lengths	109.191, 198.192, 51.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.100 - 3.200
R-factor	0.253
Rwork	0.250
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jy1
RMSD bond length	0.009
RMSD bond angle	1.372
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.100
High resolution limit [Å]	3.200
Number of reflections	19558
<I/σ(I)>	4.7
Completeness [%]	100.0
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	0.1M Ammonium sulphate, 0.1M Hepes, 25% w/v PEG 8000