5NVL

Crystal structure of the human 4EHP-GIGYF2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-19
Detector	PSI PILATUS 6M
Wavelength(s)	1.00001
Spacegroup name	P 41 21 2
Unit cell lengths	82.550, 82.550, 148.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.888 - 2.300
R-factor	0.2072
Rwork	0.205
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jgb
RMSD bond length	0.004
RMSD bond angle	0.540
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	2.360
High resolution limit [Å]	2.300	2.300
Number of reflections	23500
<I/σ(I)>	13.2	2.06
Completeness [%]	99.8	98.3
Redundancy	11.2	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M sodium citrate pH 5.0 0.1 M magnesium chloride 12% PEG 4000