5NV9
Substrate-bound outward-open state of a Na+-coupled sialic acid symporter reveals a novel Na+-site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97858 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.590, 98.070, 54.780 |
| Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
| Resolution | 78.400 - 1.950 |
| R-factor | 0.20104 |
| Rwork | 0.199 |
| R-free | 0.24346 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 78.400 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 49833 |
| <I/σ(I)> | 6.43 |
| Completeness [%] | 99.2 |
| Redundancy | 3.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M sodium citrate pH 5, 0.2-0.5M potassium chloride, 30-40% pentaerythritol propoxylate (5/4/PO/OH) |
