5NUP
Structural basis for maintenance of bacterial outer membrane lipid asymmetry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.377, 145.240, 232.535 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.383 - 2.900 |
| R-factor | 0.2432 |
| Rwork | 0.241 |
| R-free | 0.29050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1osm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.368 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 145.200 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.068 | 0.408 |
| Number of reflections | 70200 | 4460 |
| <I/σ(I)> | 7 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7 |
| CC(1/2) | 0.996 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.04 M magnesium chloride hexahydrate, 0.05 M sodium chloride, 0.1 M HEPES pH7.5 and 32 % PEG400 |
