5NUL

CLOSTRIDIUM BEIJERINCKII FLAVODOXIN MUTANT: G57T SEMIQUINONE (150K)

Experimental procedure

Temperature [K]	150
Detector technology	AREA DETECTOR
Collection date	1994-12
Spacegroup name	P 31 2 1
Unit cell lengths	60.880, 60.880, 69.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.183
Rwork	0.183
RMSD bond length	0.009
RMSD bond angle	22.840 *
Data reduction software	SDMS (DETECTOR SYSTEM (NIELSEN))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.600
Rmerge	0.068 *
Number of reflections	18988
Completeness [%]	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.5-2.8 (M)
2	1	reservoir	Tris-HCl		or phosphate