5NUI
Crystal structure of SIVmac239 Nef in an ExxxLM endocytic sorting motif bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9778 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.480, 140.730, 106.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.018 - 2.500 |
| R-factor | 0.1995 |
| Rwork | 0.195 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ik5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS ((dev_2685: ???)) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2685: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.018 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.900 |
| Number of reflections | 13775 | 794 |
| <I/σ(I)> | 20 | 2.08 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 10% PEG 4000, 0.15 M ammonium sulfate, 0.1 M MES (pH 5.8) |
