5NUE
Cytosolic Malate Dehydrogenase 1 (peroxide-treated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980105 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 64.598, 118.374, 148.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.561 - 1.350 |
| R-factor | 0.147967919778 |
| Rwork | 0.146 |
| R-free | 0.17951 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.413 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.561 | 1.398 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmeas | 0.109 | 2.934 |
| Rpim | 0.798 | |
| Number of reflections | 236503 | |
| <I/σ(I)> | 5.56 | 0.63 |
| Completeness [%] | 100.0 | 99.98 |
| Redundancy | 13.6 | 13.4 |
| CC(1/2) | 0.973 | 0.369 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 283 | 1.5 M ammonium sulfate, 0.1 M Tris-HCl |
