5NU2
Structure of non-fluorescent human urine RBP4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 102.680, 102.680, 73.180 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.500 |
R-factor | 0.15783 |
Rwork | 0.156 |
R-free | 0.18362 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5nty |
RMSD bond length | 0.004 |
RMSD bond angle | 0.702 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.490 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.037 | 0.443 |
Number of reflections | 44537 | |
<I/σ(I)> | 10.5 | 1.8 |
Completeness [%] | 96.7 | 96.3 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4.3 M NaCl, 100 mM Hepes, pH 7.5 |