5NTF
apo Structure of Leucyl aminopeptidase from Trypanosoma cruzi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 161.419, 161.419, 205.107 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.140 - 2.300 |
| R-factor | 0.21152 |
| Rwork | 0.209 |
| R-free | 0.25906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ntg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.402 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.140 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.181 | 0.987 |
| Number of reflections | 43976 | |
| <I/σ(I)> | 9.1 | 2.4 |
| Completeness [%] | 96.2 | 100 |
| Redundancy | 8.7 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Citric acid pH 4.0, 1.6 M (NH4)2SO4, 1 mM MnCl2, 5 mM actinonin |
