5NT5
The effect of Berenil and Cacodylateon the crystal structure of d(CGTGAATTCACG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2017-04-05 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.044, 41.534, 64.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.980 - 2.300 |
| R-factor | 0.24765 |
| Rwork | 0.245 |
| R-free | 0.31207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d29 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.733 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.980 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 3731 |
| <I/σ(I)> | 4 |
| Completeness [%] | 100.0 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 290 | 60mM Sodium cacodylate, 17mM MgCl2, 2m M Spermine, 50% MPD, 1.5mM Berenil |
