5NSJ
GP1 receptor-binding domain from Whitewater Arroyo mammarenavirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.377 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 128.024, 128.352, 45.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.321 - 2.249 |
| R-factor | 0.1862 |
| Rwork | 0.183 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kas |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.138 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (is phenix, but edited to fix R-factor: AC) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.310 |
| High resolution limit [Å] | 2.249 | 2.249 |
| Rmerge | 0.969 | |
| Number of reflections | 35366 | |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 98.7 | 87.6 |
| Redundancy | 6 | |
| CC(1/2) | 0.997 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris HCL pH 8.7, 0.2 M ammonium acetate, 33% w/v polyethylene glycol 3,350, 5% ethylene glycol, 3% 1,1,1,3,3,3-hexafluro-2-propanol, 50 mM sodium bromide |
