5NSE

BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE, H4B-FREE, HYDROXY-ARG COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-07-15
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	57.540, 106.170, 155.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.196
Rwork	0.196
R-free	0.24300
Structure solution method	OTHER
RMSD bond length	0.007
RMSD bond angle	1.388
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	76092
<I/σ(I)>	11.1	2.6
Completeness [%]	99.4	97.6
Redundancy	3.8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.5