5NRP

Beta domain of human transcobalamin bound to cobinamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	59.859, 59.859, 70.114
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.683 - 1.569
R-factor	0.1802
Rwork	0.179
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.083
RMSD bond angle	4.391
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.680
High resolution limit [Å]	1.570
Number of reflections	19877
<I/σ(I)>	16.79
Completeness [%]	95.5
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	0.1 M sodium malonate pH 7.0, 12% w/v polyethylene glycol 3,350