5NR4
Crystal structure of Clasp2 TOG1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.083, 47.116, 74.152 |
| Unit cell angles | 90.00, 115.83, 90.00 |
Refinement procedure
| Resolution | 38.491 - 1.198 |
| R-factor | 0.2005 |
| Rwork | 0.200 |
| R-free | 0.21360 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.273 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((dev_2420: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.500 |
| High resolution limit [Å] | 1.190 |
| Number of reflections | 128081 |
| <I/σ(I)> | 13.42 |
| Completeness [%] | 99.0 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30mM Sodium fluoride; 30mM Sodium bromide; 30mM Sodium iodide; 100mM Tris(Base)/Bicine pH 8.5; 12.5% v/v MPD; 12.5% PEG1000; 12.5% PEG3350 |
