5NQZ
Structure of a fHbp(V1.1):PorA(P1.16) chimera. Fusion at fHbp position 309.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92818 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.370, 76.270, 83.900 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 58.550 - 1.630 |
| R-factor | 0.16353 |
| Rwork | 0.162 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ayd |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.975 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.550 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.062 | 0.615 |
| Rpim | 0.041 | 0.534 |
| Number of reflections | 70772 | 4967 |
| <I/σ(I)> | 12.7 | 1.5 |
| Completeness [%] | 98.6 | 93.9 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 294 | 0.01M zinc chloride, 0.1M sodium acetate, 20%(w/v) PEG6000 |
