5NQM
CU(I)-CSP3 (COPPER STORAGE PROTEIN 3) FROM METHYLOSINUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.3746 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 44.650, 106.680, 45.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.620 - 1.590 |
| R-factor | 0.1527 |
| Rwork | 0.150 |
| R-free | 0.20655 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5arm |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.712 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.620 | 1.620 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.083 | 0.382 |
| Number of reflections | 14862 | 702 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM MgCl2, 100 mM Hepes pH 7.5, 30% PEG 400 |
