5NPW

Structure of human ATG5-ATG16L1(ATG5BD) complex (C2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	200.860, 75.990, 142.440
Unit cell angles	90.00, 131.41, 90.00

Refinement procedure

Resolution	48.316 - 3.100
R-factor	0.2198
Rwork	0.217
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gdk
RMSD bond length	0.003
RMSD bond angle	0.504
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.316	48.316	3.200
High resolution limit [Å]	3.100	10.000	3.100
Rmerge	0.126	0.037	0.745
Rmeas	0.147	0.043	0.865
Number of reflections	29428	929	2643
<I/σ(I)>	9.48	27.66	2.08
Completeness [%]	99.1	96.7	99.3
Redundancy	3.698	3.771	3.841
CC(1/2)	0.992	0.997	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4 M KCl and 18 % PEG 3350