5NPJ
Structure of the Hepatitis C virus strain JFH1 glycoprotein E2 antigenic region 532-540 bound to the single chain variable fragment of the non-neutralizing antibody DAO5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 155.689, 155.689, 61.647 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.330 - 1.900 |
| R-factor | 0.1868 |
| Rwork | 0.186 |
| R-free | 0.20170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | single chain variable fragment |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.230 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.137 | 0.614 |
| Number of reflections | 58977 | 8629 |
| <I/σ(I)> | 9.5 | 3.2 |
| Completeness [%] | 98.5 | 99.8 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES, 28% PEG400, 100mM sodium acetate |
