5NO5
AbyA5 Wildtype
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 65 |
| Detector technology | PIXEL |
| Collection date | 2013-08-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97887 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.229, 88.360, 131.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.300 - 2.500 |
| R-factor | 0.21079 |
| Rwork | 0.208 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ywf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.714 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.930 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 27564 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 96.6 |
| Redundancy | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | Wild type AbyA5 data sets were collected from crystals formed in formed in 12.5 % w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.02 M sodium L-glutamate, 0.02 M DL-alanine, 0.02 M glycine, 0.02 M DL-lysine, HCl, 0.02 M DL-serine, 0.1 M Bicine/Trizma base pH 8.5 |
