5NNZ
Crystal structure of human ODA16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.150, 69.500, 102.150 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 57.461 - 2.651 |
| R-factor | 0.2304 |
| Rwork | 0.226 |
| R-free | 0.28360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mzh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.075 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1647) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.190 | |
| Rpim | 1.350 | |
| Number of reflections | 18102 | 2798 |
| <I/σ(I)> | 5.7 | 1 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 6 | 5.9 |
| CC(1/2) | 0.980 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 285 | 50mM Tris pH 8 and 50mM NaOxalate |
