5NNX

TEAD1 bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97242
Spacegroup name	H 3 2
Unit cell lengths	112.300, 112.300, 157.562
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.000 - 3.290
R-factor	0.2032
Rwork	0.196
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z8e
RMSD bond length	0.032
RMSD bond angle	1.650
Data reduction software	XDS
Data scaling software	Aimless (0.5.25)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.460	46.460	3.550
High resolution limit [Å]	3.290	8.700	3.290
Rmerge	0.079	0.025	2.218
Rmeas	0.087	0.027	2.442
Rpim	0.035	0.011	0.992
Number of reflections	5806
<I/σ(I)>	9.8
Completeness [%]	97.4	95.2	93.6
Redundancy	5.9	5.3	5.7
CC(1/2)	1.000	1.000	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.24	298	Ammonium Sulphate, MOPS, PEG (6000), PME(550)