5NNS
Crystal structure of HiLPMO9B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.695, 46.375, 86.492 |
| Unit cell angles | 90.00, 103.23, 90.00 |
Refinement procedure
| Resolution | 46.380 - 2.100 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.25143 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eis |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.225 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.380 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.147 | |
| Number of reflections | 24456 | |
| <I/σ(I)> | 7.2 | 1.4 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 3.7 | 3 |
| CC(1/2) | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | polyacrylic acid, MgCl2, HEPES |
