5NMJ

Chicken GRIFIN (crystallisation pH: 6.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	80.777, 80.777, 39.149
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.389 - 1.399
R-factor	0.1686
Rwork	0.167
R-free	0.20020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nld
RMSD bond length	0.005
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.390	1.450
High resolution limit [Å]	1.399	1.399
Rmerge	0.029	0.530
Number of reflections	26072	2502
<I/σ(I)>	14.45	1.36
Completeness [%]	99.7	98.08
Redundancy	2	2
CC(1/2)	0.999	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	30% PEG 400, 0.1 M MES pH 6.5, 0.1 M Sodium Acetate