5NMH
HLA A02 presenting SLYNTIATL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.120, 80.090, 57.580 |
| Unit cell angles | 90.00, 115.75, 90.00 |
Refinement procedure
| Resolution | 31.700 - 1.550 |
| R-factor | 0.16677 |
| Rwork | 0.165 |
| R-free | 0.19587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.886 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.700 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.066 | 0.733 |
| Number of reflections | 66024 | 4752 |
| <I/σ(I)> | 12.2 | 2.4 |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Sodium Cacodylate, pH 6.0, 25% PEG 4000, 0.2 M Ammonium Sulphate |
