5NM7
Crystal structure of Burkholderia AP3 phage endolysin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-06-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 154.615, 154.615, 125.823 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.720 - 1.720 |
| R-factor | 0.2097 |
| Rwork | 0.208 |
| R-free | 0.23528 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.403 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.720 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.126 | 0.684 |
| Number of reflections | 61070 | |
| <I/σ(I)> | 11.1 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 8.9 |
| CC(1/2) | 0.993 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M SPG pH 7.0, 25% PEG 1500 |
