5NM1

Chicken GRIFIN (crystallisation pH: 6.2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	P 2 21 21
Unit cell lengths	44.873, 103.930, 129.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.223 - 2.099
R-factor	0.2164
Rwork	0.213
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nld
RMSD bond length	0.007
RMSD bond angle	0.963
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.223	2.170
High resolution limit [Å]	2.099	2.099
Rmerge	0.048	0.470
Number of reflections	35607	3189
<I/σ(I)>	6.72	1.24
Completeness [%]	98.2	89.91
Redundancy	2	1.9
CC(1/2)	0.997	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	295	20% PEG 1K, 0.1M Na/K Phosphate pH 6.2, 0.2M NaCl