5NLY

Brag2 Sec7-PH (390-763), P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.872899
Spacegroup name	P 21 21 21
Unit cell lengths	63.893, 66.039, 206.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.990 - 2.000
R-factor	0.209
Rwork	0.208
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nlv
RMSD bond length	0.012
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.990	2.050
High resolution limit [Å]	2.000	2.000
Rpim	0.050	0.860
Number of reflections	60129
<I/σ(I)>	10.1	1.8
Completeness [%]	99.7
Redundancy	5.9	5.4
CC(1/2)	0.997	0.439

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG20000, O.1M Tris pH=8.5