5NLX
A2A Adenosine receptor room-temperature structure determined by serial millisecond crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2016-09-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.330, 180.070, 142.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.458 - 2.140 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22920 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.157 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 2.220 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 30837 | 2842 |
| <I/σ(I)> | 13.17 | |
| Completeness [%] | 99.4 | 93.83 |
| Redundancy | 1007.4 | 8.1 |
| CC(1/2) | 0.990 | 0.120 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 291 | 0.1M sodium citrate pH 5.0, 0.05M sodium thiocyanate, 28-34% PEG400, 5 mM ZM241385, 2% (v/v) 1,6-hexanediol |
