5NKV
Crystal structure of dimeric chlorite dismutase from Cyanothece sp. PCC7425 at pH 9.0 and 293 K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-11-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.427, 53.015, 55.337 |
| Unit cell angles | 107.30, 98.54, 109.86 |
Refinement procedure
| Resolution | 19.489 - 2.000 |
| R-factor | 0.1607 |
| Rwork | 0.160 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.581 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (dev_2689) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.489 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 27988 | 1457 |
| <I/σ(I)> | 5.6 | 2.1 |
| Completeness [%] | 80.6 | 42.1 |
| Redundancy | 2.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 293 | 0.15 M MgSO4, 0.1 M MES pH 6.5, 30% PEG 3350 grown in capillary counter-diffusion set up and buffer exchanged for 0.15 M MgSO4, 0.1 M Tris HCl pH 9.0, 30% PEG 3350, all dissolved in D2O. |
